Drag & drop or click to upload a file:
Expects a tab-delimited text-file (".txt" or ".tsv") with 1, 2, or 3 columns depending on the "Enrichment method" used. Additional or unnecessary columns will simply be ignored. You can either drag and drop a file or click on the "Choose file" button to upload a file. Alternatively, you can use the copy and paste
consist of protein identifiers (or protein groups e.g. "P02407;P14127"). The Foreground is the test group, the proteins of interest you want to characterize in order to e.g. obtain overrepresented GO terms. To test for statistical significance the Foreground is compared to the Background. Typically, the Background consists of the entire genome (which is often not optimal for Proteomics data). Therefore we highly recommend using your own custom Background, since it will have the same biases the Foreground has (sample preparation, instrumentation, etc.). Intensity
(i.e. Abundance) is coupled to the Background, and any kind of abundance measure can be used e.g. copy number, iBAQ, LFQ, spectral counts, etc. This means for each protein identifier (or protein group) in the Background there should be a corresponding numeric value in the Intensity column (proteins with missing abundance data will not be ignored, but placed together into a missing values group). No Intensity values have to be provided for the Foreground. Further details are described below and on the FAQ
page. Unncessary data and parameters will be ignored.